Road Cycling World Championships | _pictogram.svg) | ||||
| 2023 |  | USA | Chloe Dygert | |
| 2022 |  | NED | Ellen van Dijk | |
| 2021 | | NED | Ellen van Dijk | |
| 2020 | | NED | Anna van der Breggen | |
| 2019 | | USA | Chloe Dygert | |
| 2018 | | NED | Annemiek van Vleuten | |
| 2017 | | NED | Annemiek van Vleuten | |
| 2016 | | USA | Amber Neben | |
| 2015 |  | NZL | Linda Villumsen | |
| 2014 |  | GER | Lisa Brennauer | |
| 2013 | | NED | Ellen van Dijk | |
| 2012 | | GER | Judith Arndt | |
| 2011 | | GER | Judith Arndt | |
| 2010 |  | GBR | Emma Pooley | |
| 2009 | | USA | Kristin Armstrong | |
| 2008 | | USA | Amber Neben | |
| 2007 | | GER | Hanka Kupfernagel | |
| 2006 | | USA | Kristin Armstrong | |
| 2005 |  | SUI | Karin Thürig | |
| 2004 | | SUI | Karin Thürig | |
| 2003 |  | ESP | Joane Somarriba | |
| 2002 |  | RUS | Zulfiya Zabirova | |
| 2001 |  | FRA | Jeannie Longo | |
| 2000 | | USA | Mari Holden | |
| 1999 | | NED | Leontien Van Moorsel | |
| 1998 | | NED | Leontien Van Moorsel | |
| 1997 | | FRA | Jeannie Longo | |
| 1996 | | FRA | Jeannie Longo | |
| 1995 | | FRA | Jeannie Longo | |
| 1994 | | USA | Karen Kurreck | |